Preliminary studies with a neurotoxin obtained from Bungarus caeruleus venom
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چکیده
منابع مشابه
Purification of a nontoxic phospholipase A2 from the venom of Indian krait (Bungarus caeruleus).
A nontoxic phospholipase A2 was purified from the venom of Indian krait (Bungarus caeruleus) by a four-step procedure involving electrophoresis, gel filtration and ion-exchange chromatography. The recovery of the enzyme activity was 37% and the purified preparation was 38 times as active as the crude venom. The purified enzyme had a molecular weight of 12,500 and the optimum pH of 7.2. The enzy...
متن کاملA cDNA sequence encoding a neurotoxin-homolog from Bungarus multicinctus.
The venoms of both Elapidae and Hydrophiidae are mixtures of proteins some of which are blockers of the rucotinic acetylcholine receptor (1). They are classified into shortneurotoxins (60 to 62 residues), long-neurotoxins (70 to 74 residues) and x-neurotoxins (66 residues) (2). Some peptides are classified as neurotoxin-homologs with unknown pharmacological activity (1). Here we present the fir...
متن کاملCrystal structure of a carbohydrate induced homodimer of phospholipase A2 from Bungarus caeruleus at 2.1A resolution.
This is the first crystal structure of a carbohydrate induced dimer of phospholipase A(2) (PLA(2)). This is an endogenous complex formed between two PLA(2) molecules and two mannoses. It was isolated from Krait venom (Bungarus caeruleus) and crystallized as such. The complete amino acid sequence of PLA(2) was determined using cDNA method. Three-dimensional structure of the complex has been solv...
متن کاملDeep coma and hypokalaemia of unknown aetiology following Bungarus caeruleus bites: Exploration of pathophysiological mechanisms with two case studies
Bungarotoxin present in Bungarus caeruleus (BC) causes life threatening respiratory muscle paralysis. Deep coma and hypokalaemia have been observed in a significant proportion of patients, but the cause is unknown. We postulate the likely mechanism behind these two phenomena. We studied clinical details of two patients admitted with deep coma and performed electroencephalograms (EEG) and brain ...
متن کاملMolecular Characterization of a Three-disulfide Bridges Beta-like Neurotoxin from Androctonus crassicauda Scorpion Venom
Scorpion venom is the richest source of peptide toxins with high levels of specific interactions with different ion-channel membrane proteins. The present study involved the amplification and sequencing of a 310-bp cDNA fragment encoding a beta-like neurotoxin active on sodium ion-channel from the venom glands of scorpion Androctonus crassicauda belonging to the Buthidae family using r...
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ژورنال
عنوان ژورنال: Journal of Venomous Animals and Toxins including Tropical Diseases
سال: 2006
ISSN: 1678-9199
DOI: 10.1590/s1678-91992006000100007